Nitrogen-vacancy centers in diamond for nanoscale magnetic resonance imaging applications

• Alberto Boretti,
• Lorenzo Rosa,
• Jonathan Blackledge and
• Stefania Castelletto

Beilstein J. Nanotechnol. 2019, 10, 2128–2151, doi:10.3762/bjnano.10.207

• -selective addressing (Rabi oscillations) of 1 × 4 arrays of NV sites separated by ≈15 nm. The order of three NV centers per site have the same orientation, detected by measuring the fluorescence count rates in a standard confocal volume [43]. Stimulated emission depletion (STED) microscopy (see a

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging

• Joseph R. Pyle and
• Jixin Chen

Beilstein J. Nanotechnol. 2017, 8, 2296–2306, doi:10.3762/bjnano.8.229

• as stimulated emission depletion (STED) microscopy [26][27]; (2) using software to super-localize single molecules [28][29][30][31], such as stochastic optical reconstruction microscopy (STORM) [32], photo-activated localization microscopy (PALM) [33], single-molecule high-resolution imaging with

Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy

• Isabella Tavernaro,
• Christian Cavelius,
• Henrike Peuschel and
• Annette Kraegeloh

Beilstein J. Nanotechnol. 2017, 8, 1283–1296, doi:10.3762/bjnano.8.130

• ; organic fluorescent dyes; silica nanoparticles; stimulated emission depletion (STED) microscopy; Introduction With the emergence of nanotechnology, numerous applications have been developed using the size-related properties of nanoparticles [1][2]. In biomedical research, nanoparticles are applied as

• depletion (STED) microscopy. Our approach allows for a step-by-step formation of dye-doped silica nanoparticles in the form of dye-incorporated spheres, which can be used as versatile fluorescent probes in confocal and STED imaging. Keywords: bioimaging; confocal microscopy; multistep synthesis approach

• other hand, are suitable for observing dynamic processes using living cells [18][19][20]. In addition, the development of novel so-called “super-resolution” optical imaging techniques allows for the imaging of objects beyond the diffraction limit [21][22]. For example, stimulated emission depletion

Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy

• Adriano A. Torrano and
• Christoph Bräuchle

Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173

• to be accurate by independent stimulated emission depletion (STED) microscopy, a super-resolution technique [28][29]. Although developed for the absolute quantification of the nanoparticle uptake by cells, this method was made flexible to allow for the quantification in absolute and also in relative